AIFM3 Knockout cell line (Hep G2)

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AIFM3 Knockout cell line (Hep G2) Order

AIFM3 Knockout cell line (Hep G2)
Catalog#
YKO-HT4243
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
AIFM3 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
AIFM3 Knockout cell line (Hep G2)
Species
Human
Gene Name
AIFM3
Gene ID
150209
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 3 (based on transcript AIFM3-207)
Validation: PCR+Sanger Sequencing
AIFM3 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the AIFM3 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, AIFM3 gene knockout AIFM3 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
AIFM3 Knockout cell line (Hep G2)
AIFM3 Knockout cell line (Hep G2)
Catalog#
YKO-HT4243
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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Gene Knockout kit

EZ-cryo™ cell freezing medium

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  • AIFM3 Knockout cell line (293T)

    AIFM3 Knockout cell line (293T)

    Organism: Human
    Gene Name: AIFM3
    Gene ID: 150209
    Size: 1*10^6
    Catalog#: YKO-H1076
    YKO-H1076(293T)
  • AIFM3 Knockout cell line (HCT 116)

    AIFM3 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: AIFM3
    Gene ID: 150209
    Size: 1*10^6
    Catalog#: YKO-XN72931
    YKO-XN72931(HCT 116)
  • AIFM3 Knockout cell line (A549)

    AIFM3 Knockout cell line (A549)

    Organism: Human
    Gene Name: AIFM3
    Gene ID: 150209
    Size: 1*10^6
    Catalog#: YKO-XN72929
    YKO-XN72929(A549)

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Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium

KO Cell Lines

  • AIFM3 Knockout cell line (293T)

    AIFM3 Knockout cell line (293T)

    Organism: Human
    Gene Name: AIFM3
    Gene ID: 150209
    Size: 1*10^6
    Catalog#: YKO-H1076
  • AIFM3 Knockout cell line (HCT 116)

    AIFM3 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: AIFM3
    Gene ID: 150209
    Size: 1*10^6
    Catalog#: YKO-XN72931
  • AIFM3 Knockout cell line (A549)

    AIFM3 Knockout cell line (A549)

    Organism: Human
    Gene Name: AIFM3
    Gene ID: 150209
    Size: 1*10^6
    Catalog#: YKO-XN72929

AIFM3 Knockout cell line (Hep G2) Order

AIFM3 Knockout cell line (Hep G2)
Catalog#
YKO-HT4243
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
AIFM3 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
AIFM3 Knockout cell line (Hep G2)
Species
Human
Gene Name
AIFM3
Gene ID
150209
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 3 (based on transcript AIFM3-207)
Validation: PCR+Sanger Sequencing
AIFM3 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the AIFM3 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, AIFM3 gene knockout AIFM3 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
AIFM3 Knockout cell line (Hep G2)
AIFM3 Knockout cell line (Hep G2)
Catalog#
YKO-HT4243
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • AIFM3 Knockout cell line (293T)

    AIFM3 Knockout cell line (293T)

    Organism: Human
    Gene Name: AIFM3
    Gene ID: 150209
    Size: 1*10^6
    Catalog#: YKO-H1076
    YKO-H1076(293T)
  • AIFM3 Knockout cell line (HCT 116)

    AIFM3 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: AIFM3
    Gene ID: 150209
    Size: 1*10^6
    Catalog#: YKO-XN72931
    YKO-XN72931(HCT 116)
  • AIFM3 Knockout cell line (A549)

    AIFM3 Knockout cell line (A549)

    Organism: Human
    Gene Name: AIFM3
    Gene ID: 150209
    Size: 1*10^6
    Catalog#: YKO-XN72929
    YKO-XN72929(A549)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium

KO Cell Lines

  • AIFM3 Knockout cell line (293T)

    AIFM3 Knockout cell line (293T)

    Organism: Human
    Gene Name: AIFM3
    Gene ID: 150209
    Size: 1*10^6
    Catalog#: YKO-H1076
  • AIFM3 Knockout cell line (HCT 116)

    AIFM3 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: AIFM3
    Gene ID: 150209
    Size: 1*10^6
    Catalog#: YKO-XN72931
  • AIFM3 Knockout cell line (A549)

    AIFM3 Knockout cell line (A549)

    Organism: Human
    Gene Name: AIFM3
    Gene ID: 150209
    Size: 1*10^6
    Catalog#: YKO-XN72929

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